![]() ![]() Please check the code for the calculation of ppm. Only 1H spectrum is more or less correct, but peak is still a bit out of 2.00 ppm.įor 13C spectrum misalignment is dramatic.īy the way Mnova also displays incorrectly 2D spectrum. projections are not correlated with 2D spectrum, 2. ![]() Then I load these spectra into CCPN analysisĪnd now I see that 1. Just for fun I calibrated 1H peak to 2.000ppm, 13C peak 20.000, and also in HSQC 2.0 and 20.0 ppm, respectively. Found strong and narrow CH3 peak (Acetate ion) and calibrated it in all three spectra using TOPSPIN (so SR is not zero anymore). Measured three spectra: 1H, 13C, and 13C-1H HSQC. Especially in HSQC and 1D 13C spectra. Incorrect means, that CCPN place my peak few hundreds away from where I want. I faced the problem of incorrect display of the chemical shifts on the spectra calibrated in Topspin. I just installed CCPN V3.02 for assignment of short peptide. ![]()
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